Thermo Fisher Scientific Ly-49H Monoclonal Antibody (3D10), Biotin, eBioscience
Ly-49H 단일클론 항체(3D10), Biotin 결합형으로 마우스 NK 세포의 Ly-49H 수용체를 특이적으로 인식합니다. Flow Cytometry에 최적화되어 있으며, 0.5 mg/mL 농도의 액상 제품입니다. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.125 µg/test | View 5 publications |
| Neutralization (Neu) | - | View 1 publication |
| Functional Assay (Functional) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3D10 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_906243 |
Available Formats
Product Specific Information
The monoclonal antibody 3D10 recognizes Ly-49H, a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer (NK) cells.
Ly-49H, similar to Ly-49D, lacks the ITIM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine residue in the transmembrane domain, suggesting it functions as an activation receptor.
Ly-49H is expressed in a subset of NK cells but not in NKT (NK1.1⁺CD3⁺) cells. NK cells can express multiple Ly-49 proteins. Preferential staining of Ly-49H with Ly-49D has been observed. Expression is confirmed in C57BL/6 and NWNA mice, but not in Balb/c or DBA/2 strains. No cross-reactivity with Ly-49A, C, D, or G2 has been detected.
Addition of monoclonal antibody 3D10 to cell cultures induces lysis of target cells.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on C57Bl/6 spleen cells; usable at ≤1 µg/test (in 100 µL final volume).
Cell numbers can vary from 10⁵–10⁸ cells/test. Optimize antibody titration for best results.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Ly49H is a C-type lectin family receptor on NK cells.
It lacks ITAM and contains an arginine residue in the transmembrane domain, functioning as an activation receptor.
Expressed in NK cells but not in NKT (NK1.1/CD3⁺) cells. NK cells may express multiple Ly49 receptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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