Thermo Fisher Scientific Ly-49G Monoclonal Antibody (AT-8 (AT8)), Biotin, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

AT-8 (AT8)

Conjugate

Biotin Biotin Biotin

View additional formats

• PE
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466754

Product Specific Information

Description: The AT8 monoclonal antibody reacts with the mouse Ly-49G receptor from 129, FVB, BALB/c, C3H, CBA, SJL and A/J, but not C57Bl/6 mice. Ly49G, a member of the Ly49 family is expressed by NK cell subset.

Applications Reported: The AT-8 (AT8) antibody has been reported for use in flow cytometric analysis.

Applications Tested: The AT-8 (AT8) antibody has been tested by flow cytometric analysis of BALB/c mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated. These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.