Thermo Fisher Scientific CD314 (NKG2D) Monoclonal Antibody (A10), Biotin, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test
• Publications: View 1 publication

Product Specifications

Product Specific Information

The A10 monoclonal antibody reacts with mouse NKG2D, a lectin-like molecule expressed on NK cells. Mouse NKG2D binds to RAE-1 isoforms and H60, functioning as a costimulatory receptor through DAP12/DAP10 adaptors.
NKG2D is expressed by spleen and liver NK cells, NK1.1(+) thymocytes, activated LAK cells, and subsets of splenic NKT cells. A10 and C7 antibodies detect NK cells from all tested mouse strains.

A10 can stimulate NK cells to lyse Daudi targets and induce GM-CSF production when immobilized. A10 and C7 bind similar epitopes but differ functionally (A10 activating, C7 neutralizing).
Expression of NKG2D can be detected by flow cytometry using mAb CX5 with higher intensity.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes; recommended ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize concentration for best performance.

Target Information

Natural killer (NK) cells mediate lysis of tumor and virus-infected cells without prior activation and regulate immune responses. They express C-type lectins, including NKG2 family members.
The NKG2 gene encodes a type II transmembrane protein with a C-type lectin domain, located in the NK complex region containing NK-specific lectin genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.