Thermo Fisher Scientific c-Myc Recombinant Rabbit Monoclonal Antibody (27H46L35)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generates a ~25 kDa light chain band and a ~50 kDa heavy chain.
• Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning antibody DNA sequences from immunoreactive rabbits.
• Advantages include:
  • Improved specificity and sensitivity
  • Lot-to-lot consistency
  • Animal origin-free formulation
  • Broader immunoreactivity due to larger rabbit immune repertoire

Target Information

The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is frequently activated in various tumor cells and plays key roles in cellular proliferation, differentiation, apoptosis, and cell cycle progression.

Phosphorylation at Thr58/Ser62 by kinases such as GSK3, CDK, ERK2, and JNK is associated with regulation of cell proliferation. c-Myc is essential for vasculogenesis and angiogenesis during tumor development.

The c-myc oncogene (p62 c-myc) regulates normal cellular proliferation and differentiation, while deregulated expression can induce apoptosis. Antibodies against c-Myc epitopes recognize Myc-tagged fusion proteins.

Amplification or translocation of the c-Myc gene has been identified in several human cancers, including lung, breast, colon, and Burkitt’s lymphoma, as well as other B-cell lymphoproliferative disorders. Aberrant c-Myc expression occurs in tumors of diverse origins such as colorectal, gastric, hepatic, ovarian, and hematopoietic neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.