Thermo Fisher Scientific Digoxigenin Recombinant Rabbit Monoclonal Antibody (9H27L19)
Digoxigenin에 특이적인 Thermo Fisher Scientific의 재조합 토끼 단클론 항체로 높은 특이성과 민감도를 제공. 다양한 응용 분야(WB, IHC, ICC, Flow, ELISA, ISH)에 사용 가능. 로트 간 일관성과 동물 유래 성분이 없는 포뮬레이션으로 연구 재현성 향상.
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Thermo Fisher Scientific Digoxigenin Recombinant Rabbit Monoclonal Antibody (9H27L19)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | – | View 4 publications |
| Immunocytochemistry (ICC/IF) | – | View 2 publications |
| Flow Cytometry (Flow) | – | View 1 publication |
| ELISA | – | View 2 publications |
| In Situ Hybridization (ISH) | – | View 2 publications |
| Miscellaneous PubMed (Misc) | – | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Published Species | Chemical, Fungi, Mouse, Rat, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 9H27L19 |
| Immunogen | Proprietary |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532342 |
Product Specific Information
- Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generates a ~25 kDa light chain band and a ~50 kDa heavy chain.
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems.
- Expression systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits.
- Individual clones are screened to select the best candidates for production.
- Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Digoxin, also known as digitalis, is a purified cardiac glycoside extracted from Digitalis lanata.
Its aglycone is digoxigenin, and its acetyl derivative is acetyldigoxin.
Digoxin is widely used in the treatment of heart conditions such as atrial fibrillation, atrial flutter, and heart failure.
It is marketed under trade names Lanoxin, Digitek, and Lanoxicaps.
Digoxin is also used as an affinity system for nonradioactive labeling and detection of proteins and nucleic acids.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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