Thermo Fisher Scientific GATA1 Recombinant Rabbit Monoclonal Antibody (4H15L5)
GATA1 단백질을 인식하는 토끼 유래 재조합 단클론 항체로, Western blot과 면역세포염색에 적합합니다. 일관된 로트 간 품질, 높은 특이성과 민감도를 제공합니다. 단백질 발현 및 적혈구 발달 연구용으로 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–3 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100 | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 4H15L5 |
| Immunogen | Recombinant protein corresponding to amino acids 1–200 of human GATA1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532341 |
Product Specific Information
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
GATA1 is a transcriptional activator or repressor that functions as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5′-[AT]GATA[AG]-3′ within regulatory regions of globin genes and other genes expressed in erythroid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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