Thermo Fisher Scientific SHANK1 Monoclonal Antibody (N22/21)
SHANK1 단백질을 인식하는 마우스 모노클로날 항체로, WB, IHC(P), Antibody Microarray에 사용 가능. 인간, 마우스, 랫트 반응성. Protein G 정제, 1 mg/mL 농도. 신경 시냅스 단백질 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N22/21 |
| Immunogen | Fusion protein amino acids 469–691 (SH3/PDZ domains) of rat Shank1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735360 |
Additional Formats Available
Product Specific Information
1 µg/mL of MA5-27626 was sufficient for detection of Shank1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 190–220 kDa (alternative splice variants). No cross-reactivity against Shank2 or Shank3.
Target Information
SHANK1 is an adapter protein in the postsynaptic density (PSD) of excitatory synapses. It interconnects receptors of the postsynaptic membrane, including NMDA-type and metabotropic glutamate receptors, via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. It may play a role in the structural and functional organization of dendritic spines and synaptic junctions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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