
Thermo Fisher Scientific Protocadherin Gamma Monoclonal Antibody (N159/5)
Protocadherin Gamma 단백질을 검출하기 위한 Mouse monoclonal 항체로, WB, IHC, ICC/IF에 사용 가능. Human, Mouse, Rat에 반응하며, 단일 클론 N159/5 사용. 단백질 G 정제, PBS/glycerol buffer에 보관, -20°C 저장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N159/5 |
| Immunogen | Fusion protein corresponding to amino acids 808–931 of mouse Protocadherin-gamma-A1 (C-terminal cytoplasmic constant domain), shared by all 22 Gamma-protocadherins. 99% identity with human. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735332 |
Available Formats
Product Specific Information
- 1 µg/mL of MA5-27615 was sufficient for detection of Protocadherin gamma (pan) in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Detects approximately 100 kDa.
- Cross-reacts with all Gamma-protocadherin-A, -B, and -C proteins.
- This antibody was formerly sold as clone S159-5.
Target Information
The protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. These clusters have an immunoglobulin-like organization, suggesting a novel mechanism for regulation and expression.
The gamma cluster includes 22 genes divided into 3 subfamilies:
- Subfamily A: 12 genes
- Subfamily B: 7 genes and 2 pseudogenes
- Subfamily C: 3 genes
Each variable region exon encodes the extracellular region with 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic domain. These neural cadherin-like proteins likely play a critical role in establishing specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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