Thermo Fisher Scientific TIGIT Monoclonal Antibody (GIGD7), eFluor 660, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 650
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 702
• Request custom conjugation

Product Specific Information

The GIGD7 monoclonal antibody reacts with mouse TIGIT (T cell immunoreceptor with Ig and ITIM domains, also known as VSTM3 or WUCAM), a 26 kDa protein belonging to the poliovirus receptor (PVR) family.
In mice, TIGIT is expressed on follicular T helper cells, while in humans, it is expressed on NK cells, regulatory T cells, follicular T helper cells, memory CD4+ T cells, and CD8+ T cells. TIGIT is not expressed on B cells or naïve CD4+ T cells but is upregulated upon activation.

TIGIT interacts with members of the PVR and PVR-like families (e.g., CD155) and mediates interactions between NK/T cells and antigen-presenting cells, fibroblasts, and endothelial cells expressing PVR-like proteins.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤1 µg/test (100 µL final volume). Optimal titration recommended.

Fluorophore Information:
eFluor® 660 replaces Alexa Fluor® 647.

• Excitation: 633–647 nm
• Emission: 668 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Binds with high affinity to the poliovirus receptor (PVR), leading to increased IL10 and decreased IL12B secretion, suppressing T cell activation by promoting mature immunoregulatory dendritic cell generation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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