Thermo Fisher Scientific IDO Monoclonal Antibody (eyedio), eFluor 660, eBioscience

Thermo Fisher Scientific IDO Monoclonal Antibody (eyedio), eFluor 660, eBioscience™

Applications and Tested Dilution

Publications

• View 6 publications

Product Specifications

Available Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 395
• Brilliant UV 615
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 786
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This eyedio monoclonal antibody reacts with human indoleamine-2,3-dioxygenase (IDO, INDO, IDO1), an intracellular enzyme that catalyzes the degradation of tryptophan to kynurenines. IDO is expressed in various tissues and cell types, including macrophages and dendritic cells, and can be induced by IFN-γ or other inflammatory stimuli.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated and validated using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) according to BestProtocols®: Protocol A.
Use at 5 µL (0.06 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Fluorophore Information:
eFluor™ 660 replaces Alexa Fluor™ 647, emits at 659 nm, and is excited by the red laser (633 nm).

• Excitation: 633–647 nm
• Emission: 668 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

IDO1 is a heme-containing enzyme that catalyzes oxidative cleavage of the indole ring in molecules such as tryptophan, serotonin, and melatonin, producing biologically active kynurenines. It is widely expressed in human tissues, macrophages, and dendritic cells. IFN-induced IDO1 expression mediates antimicrobial and immunoregulatory effects, influencing infection, pregnancy, autoimmunity, and tumor immunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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