Thermo Fisher Scientific IDO Monoclonal Antibody (mIDO-48), eFluor 660, eBioscience
마우스 IDO1 단백질을 인식하는 mIDO-48 단클론 항체로, eFluor 660 형광 표지. Flow cytometry, WB, IHC, ICC 등 다양한 응용에 적합. 4°C 암소 보관, 동결 금지. 연구용 전용 제품.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IDO Monoclonal Antibody (mIDO-48), eFluor 660, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | 0.4 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.2 µg/mL |
| Flow Cytometry (Flow) | 1 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | mIDO-48 |
| Conjugate | eFluor™ 660 |
| Excitation/Emission Max | 651/668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574334 |
Additional Formats
Product Specific Information
Description:
The monoclonal antibody mIDO-48 reacts with mouse indoleamine 2,3-dioxygenase 1 (IDO1). IDO1 is an intracellular enzyme expressed by dendritic cells, interferon gamma-activated macrophages, epithelial cells, vascular endothelium, and tumor cells. It catalyzes the conversion of tryptophan into kynurenine metabolites, which suppress T cell proliferation and promote regulatory T cell activation.
Applications Reported:
This antibody is reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested:
Tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00).
Refer to BestProtocols®: Protocol A for cytoplasmic intracellular proteins.
Use ≤1 µg per test (defined as antibody amount per 100 µL cell sample). Cell number: 10⁵–10⁸ cells/test. Optimize antibody titration for best performance.
Fluorochrome Information:
eFluor® 660 replaces Alexa Fluor® 647.
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
IDO1 is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring in tryptophan, serotonin, and melatonin, producing biologically active kynurenines. It is expressed in various tissues, macrophages, and dendritic cells. Interferons (IFNs) induce IDO1 expression, contributing to antimicrobial defense by depleting tryptophan, thereby inhibiting viral, bacterial, and parasitic growth. IDO1 also plays roles in immune regulation during infection, pregnancy, autoimmunity, transplantation, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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