Thermo Scientific™

GeneJET PCR Purification Kit

Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column,자세히 알아보기

Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions. It effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures.

The kit can be used for purification of DNA fragments from 25 bp to 20 kb with recovery rates up to 100%. Each GeneJET purification column has a total binding capacity of up to 25 μg of DNA, and the entire procedure takes 5 minutes. The purified DNA can be used in common downstream applications, such as sequencing, restriction digestion, labeling, ligation, cloning, in vitro transcription, blotting or in situ hybridization.

Highlights

• Highly efficient: 90 to 100% recoveries in the range of 100 bp to 10 kb
• Fast: procedure takes only 5 minutes
• Convenient: spin columns are capped and assembled with collection tubes

Applications

Fast and efficient purification of DNA fragments ideal for use in all conventional molecular biology procedures including:

• FastDigest or conventional restriction digestion
• Automated fluorescent or radioactive sequencing
• PCR
• In vitro transcription
• In situ hybridization
• Labeling
• Cloning

사양

형식Spin Column

고처리량 호환성Not High-throughput Compatible (Manual)

제품라인GeneJET

제품 유형PCR Purification Kit

수량50 Preps

실행 시간5 min.

타겟DNA from Enzymatic Reactions (e.g. PCR)

용도(애플리케이션)Real Time PCR (qPCR), Cloning, DNA Sequencing

분리 기술Spin Column

샘플 종류dsDNA

Unit SizeEach