Thermo Scientific™

DecaLabel DNA Labeling Kit

Thermo Scientific DecaLabel DNA Labeling Kit is an advanced system for fast synthesis of radiolabeled DNA probes of high specific자세히 알아보기

Thermo Scientific DecaLabel DNA Labeling Kit is an advanced system for fast synthesis of radiolabeled DNA probes of high specific activity. It`s based on the improved random-primed method developed by Feinberg and Vogelstein.

The primary improvement over traditional random-primer kits involves the use of random decamers instead of hexamers to ensure more efficient annealing with DNA at 37°C. In addition, Klenow Fragment, exo- has been genetically engineered to remove exonuclease activity. The improved enzyme does not degrade the labeled probe during reaction and allows high labeling yields even from low template amounts. DNA fragments of any length can be uniformly labeled. Two labeling mixes are provided for flexibility in using either radiolabeled dATP or dCTP.

Highlights

• High specific radioactivity of probes—greater than 1 x 109 dpm/μg DNA
• Fast—5 minutes at 37°C
• High yields with Klenow Fragment, exo no degradation of a labeled probe during reaction
• Flexible—suitable for either radiolabeled dATP or dCTP

Applications

• Generation of radiolabeled DNA probes for use in a variety of hybridization experiments: Southern and Northern blots, colony/plaque hybridizations, dot/slot blots, and in situ hybridizations.

Includes

• Klenow Fragment, exo-
• Decanucleotides in Reaction Buffer
• Mix A (-dATP)
• Mix C (-dCTP)
• dNTP Mix
• Control Template
• Water, nuclease-free
• Detailed Protocol

Principle
Random decamers are annealed to a denatured template DNA, and radiolabeled dNTPs are then incorporated into new DNA strands by Klenow Fragment, exo- (see Figure 1 under Supporting Data).

사양

검출 방법Radioactive

형식Kit

최종 제품 유형DNA

Labeling TargetGenomic DNA, PCR Products, DNA (General)

라벨 또는 염료 포함No

라벨링 방법Direct Labeling

제품라인DecaLabel

제품 유형DNA Labeling Kit

수량30 Reactions

Unit SizeEach