Thermo Fisher Scientific BACE1 Polyclonal Antibody
Thermo Fisher Scientific의 BACE1 Polyclonal Antibody는 인간 및 마우스 시료에 반응하는 Rabbit IgG 항체로, Western blot, ICC/IF, ELISA, IP에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 알츠하이머병 연구에서 BACE1 단백질 검출에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:200–1:2,000 | View 29 publications |
| Immunocytochemistry (ICC/IF) | 1:20–1:200 | View 5 publications |
| ELISA | Assay-dependent | View 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | View 5 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues C(485)-K(501) of human beta-secretase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_325863 |
Product Specific Information
PA1-757 detects transfected beta-secretase 1.
It has been successfully used in ELISA, ICC/IF, Western blot, and immunoprecipitation applications.
The molecular weight of BACE1 detected may vary depending on the lysate tested, which may be due to glycosylation differences among cell lines.
The PA1-757 immunizing peptide (Cat. # PEP-093) is available for neutralization and control experiments.
Target Information
BACE1 (beta-secretase) is a key enzyme involved in the production of amyloid beta-peptides (Abeta) found in extracellular amyloid plaques of Alzheimer’s disease (AD).
In some cases, early-onset familial AD can be attributed to a “Swedish” mutation in the amyloid precursor protein (APP), which enhances cleavage by BACE1.
Therapeutic approaches have focused on inhibiting BACE1 and other APP-cleaving enzymes, such as gamma-secretase.
APP is first cleaved by beta-secretase, producing a soluble derivative and a membrane-anchored 99-amino acid fragment (C99).
C99 serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide, which accumulates in brains of Alzheimer’s patients.
BACE1 (Asp2) is a transmembrane aspartic protease that colocalizes with APP.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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