Thermo Fisher Scientific BACE1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:2,000

View 29 publications 29 publications

Immunocytochemistry (ICC/IF)

1:20-1:200

View 5 publications 5 publications

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C(485) L R Q Q H D D F A D D I S L L K(501) of human beta-secretase. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:BACE1,uniProtId:P56817-1,ncbiNodeId:9606,antigenRange:485-501,antigenLength:501,antigenImageFileName:PA1-757_BACE1_P56817-1_Rabbit.svg,antigenImageFileNamePDP:PA1-757_BACE1_P56817-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325863

Product Specific Information

PA1-757 detects transfected beta-secretase 1.

PA1-757 has been successfully used in ELISA, ICC/IF, Western blot and immunoprecipitation applications. The molecular weight of BACE1 detected may vary depending on the lysate tested. This may be due to glycosylation differences in various cell lines.

The PA1-757 immunizing peptide (Cat. # PEP-093) is available for use in neutralization and control experiments.

Target Information

BACE1 (beta-secretase) is a key enzyme involved in the production of amyloid beta-peptides (Abeta) found in extracellular amyloid plaques of Alzheimers disease (AD). In some cases early onset familial AD can be attributed to aSwedishmutation in the amyloid precursor protein (APP), which dramatically enhances the cleavage of this protein by BACE1. This and other genetic and pathological evidence has led to therapeutic approaches that have focused on the inhibition of BACE1 and other APP-cleaving enzymes, such as gamma-secretase stuff. APP is first cleaved by b-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for g-secretase to generate the 4 kDa amyloid-b peptide, which is deposited in the brains of all sufferers of Alzheimers disease. The long-sought b-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). bACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.