Thermo Fisher Scientific AANAT Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

-

Immunohistochemistry (IHC)

5-10 µg/mL

-

Product Specifications

Species Reactivity

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: C(195) H T F L R R N S G C(205) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AANAT,uniProtId:Q64666-1,ncbiNodeId:10114,antigenRange:195-205,antigenLength:205,antigenImageFileName:PA1-762_AANAT_Q64666-1_Rabbit.svg,antigenImageFileNamePDP:PA1-762_AANAT_Q64666-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2273325

Product Specific Information

PA1-762 has successfully been used to detect serotonin N-AT in both WB and IF applications.

PA1-762 detects serotonin N-acetyltransferase (AA-NAT) from rat tissues.

PA1-762 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~23 kDa protein representing unphosphorylated and phosphorylated AA-NAT from rat pineal gland whole protein extract.

PA1-762 immunizing phosphopeptide (Cat. # PEP-066) corresponds to carboxy-terminal amino acid residues 198-208 of rat AA-NAT. Total anti-carboxy-terminal AA-NAT-specific IgG (Cat. # PA1-762) was affinity purified on resin-coupled non-phosphopeptide (Cat. # PEP-065). Phospho-specific carboxy-terminal IgG (Cat. # PA1-763) was subsequently isolated on resin-coupled phosphopeptide (Cat. # PEP-066). This sequence is completely conserved between rat and mouse AA-NAT protein.

Target Information

Arylalkylamine N-Acetyltransferase (AANAT) is the penultimate enzyme is melatonin synthesis. Hormonal melatonin has been implicated in sleep and circadian clock function. Large changes in the abundance of AANAT are believed to be responsible for the large day/night rhythms in melatonin synthesis in the pineal gland and retina. Most research shows that AANAT activity is regulated by controlling the steady-state levels of the protein. Recent research, however, suggests that another mechanism may also play a role in controlling melatonin production without altering AANAT protein and activity. This mechanism may explain the sudden physiological increase in circulating melatonin observed at the onset of the dark period in humans.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.