
Thermo Fisher Scientific Mouse anti-Human IgG3 (Hinge region) Secondary Antibody
Mouse monoclonal secondary antibody specific to human IgG3 hinge region. Suitable for IHC (Frozen), Flow Cytometry, and ELISA. Unconjugated form with 1 mg/mL concentration. Provides high specificity and sensitivity for research applications.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent |
| Flow Cytometry (Flow) | 10 µL for 10⁶ cells in 100 µL |
| ELISA (ELISA) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Secondary Antibody |
| Clone | HP6050 |
| Immunogen | Purified human IgG3 myeloma protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10977991 |
| Target | IgG3 |
| Antibody Form | Whole Antibody |
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting, or purification of their specified targets.
Secondary antibodies offer increased versatility, enabling users to use many detection systems (e.g., HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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