Thermo Fisher Scientific E-selectin Monoclonal Antibody (CL2/6)
인간 E-selectin을 인식하는 CL2/6 클론 단일클론 항체로, IHC, ICC/IF, Flow Cytometry에 사용 가능. 비결합형 액상 형태이며 1 mg/mL 농도로 제공. 염증 반응 연구 및 혈관 내피세포 접착 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | 0.5–2 µg/mL | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | View 1 publication |
| Flow Cytometry (Flow) | 1:20 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CL2/6 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1955403 |
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. Leukocyte adhesion also occurs in capillaries and small venules throughout the body after inflammation onset. This immediate response involves a cascade of adhesion receptors, including members of the selectin and immunoglobulin superfamilies.
E-selectin (CD62E) is an endothelial cell-specific selectin expressed only after activation with proinflammatory cytokines. IL-1, TNFα, and bacterial components such as lipopolysaccharides induce E-selectin transcription via an NFκB-dependent signaling cascade. E-selectin is associated with endothelial cells in various inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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