Thermo Fisher Scientific Mouse anti-Human IgG4 Secondary Antibody, HRP

Applications

Tested Dilution

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunohistochemistry (Frozen) (IHC (F))

1:40-1:80

ELISA (ELISA)

1:25,600-1:51,200

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Secondary Antibody

Clone

HP6025

Immunogen

Purified IgG4

Conjugate

HRP HRP HRP

Form

Liquid

Concentration

0.6 mg/mL

Purification

Protein A

Storage buffer

PBS with 1% BSA

Contains

0.002% Bromonitrodioxane, 0.002% Methylisothiazolone, 0.05% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10981963

Target

IgG4

Antibody Form

Whole Antibody

Product Specific Information

There is no cross reaction with human IgG1, IgG2 or IgG3 isotypes.

A suggested positive control for this product is tonsil.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.