Thermo Fisher Scientific Mouse anti-Human IgG4 Secondary Antibody, HRP
Human IgG4 특이적 마우스 단클론 HRP 결합 2차 항체로, IHC 및 ELISA에 최적화됨. IgG1, IgG2, IgG3과 교차 반응 없음. 단백질 A로 정제된 고순도 항체이며, 신호 증폭 및 다양한 검출 시스템에 활용 가능.
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Thermo Fisher Scientific Mouse anti-Human IgG4 Secondary Antibody, HRP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:40–1:80 |
| ELISA | 1:25,600–1:51,200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Secondary Antibody |
| Clone | HP6025 |
| Immunogen | Purified IgG4 |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA |
| Contains | 0.002% Bromonitrodioxane, 0.002% Methylisothiazolone, 0.05% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10981963 |
| Target | IgG4 |
| Antibody Form | Whole Antibody |
Product Specific Information
- No cross reaction with human IgG1, IgG2, or IgG3 isotypes.
- Suggested positive control: tonsil.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of specified targets.
Secondary antibodies offer increased versatility, enabling use with various detection systems (HRP, AP, fluorescence) and greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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