Thermo Fisher Scientific HSF1 Monoclonal Antibody (4D5F4)

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody has been tested in indirect ELISA.

Target Information

All organisms respond to elevated temperatures and various environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator HSF (Heat Shock Factor), which binds to heat shock response elements (HSEs). These HSEs consist of three repeats of a 5-nucleotide {nGAAn} module arranged in alternating orientation and located upstream of heat shock genes.

HSFs are highly conserved among species, but purified HSFs from yeast, Drosophila, and human differ in molecular weight and show little immunological cross-reactivity. Two HSFs have been identified in human cells, HSF1 and HSF2, sharing 38% sequence identity. HSF1 responds to classical stress signals such as heat, heavy metals, and oxidative agents, while HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells.

HSF1 exists constitutively in the cytoplasm and nucleus of unstressed cells as a monomer lacking DNA-binding activity. Upon stress, HSF1 is activated to a nuclear-localized, trimeric form that binds DNA. Phosphorylation of HSF1 is required for maximal transcription of heat shock genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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