Thermo Fisher Scientific HSF1 Monoclonal Antibody (4D5F4)
HSF1 단백질 검출용 Thermo Fisher monoclonal antibody. Western blot, IHC, ICC/IF, ELISA 등 다양한 응용 가능. Human 반응성, Mouse IgG2b 클론 4D5F4. 고순도 Protein G 정제, 안정적 PBS buffer 저장. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4D5F4 |
| Immunogen | Purified recombinant fragment of human HSF1 (aa 256–359) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898452 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
All organisms respond to elevated temperatures and various environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator HSF (Heat Shock Factor), which binds to heat shock response elements (HSEs). These HSEs consist of three repeats of a 5-nucleotide {nGAAn} module arranged in alternating orientation and located upstream of heat shock genes.
HSFs are highly conserved among species, but purified HSFs from yeast, Drosophila, and human differ in molecular weight and show little immunological cross-reactivity. Two HSFs have been identified in human cells, HSF1 and HSF2, sharing 38% sequence identity. HSF1 responds to classical stress signals such as heat, heavy metals, and oxidative agents, while HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells.
HSF1 exists constitutively in the cytoplasm and nucleus of unstressed cells as a monomer lacking DNA-binding activity. Upon stress, HSF1 is activated to a nuclear-localized, trimeric form that binds DNA. Phosphorylation of HSF1 is required for maximal transcription of heat shock genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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