Thermo Fisher Scientific HDAC4 Monoclonal Antibody (5E4A5)
HDAC4 단백질을 인식하는 Mouse IgG1 단일클론 항체로 인간 및 생쥐 반응성. WB, IHC, Flow, ELISA 등 다양한 응용 가능. 단백질 G 정제, 액상 형태, 1 mg/mL 농도. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5E4A5 |
| Immunogen | Purified recombinant fragment of human HDAC4 (aa: 456–592) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C and avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898449 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
Histone deacetylase 4 (HDAC4) is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3, and H4). Histone deacetylation provides an epigenetic repression tag and plays a key role in transcriptional regulation, cell cycle progression, and developmental events. HDAC4 functions via the formation of large multiprotein complexes and is involved in muscle maturation through interactions with myocyte enhancer factors (MEF2A, MEF2C, MEF2D). It also participates in MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Mutations in the HDAC4 gene have been associated with Brachydactyly-mental retardation syndrome (BDMR).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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