Thermo Fisher Scientific CD22 Monoclonal Antibody (eBio4KB128 (4KB128)), NovaFluor Blue 555, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• FITC
• NovaFluor Blue 690
• NovaFluor Red 755
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Custom conjugation available upon request

Product Specific Information

The eBio4KB128 monoclonal antibody recognizes human CD22 (Siglec-2), a member of the siglec subgroup of the Ig superfamily. CD22 is a type I transmembrane glycoprotein composed of two polypeptide chains (130 and 140 kDa) produced by alternative splicing. It is highly expressed on mature B cells and B cell lymphomas.

The extracellular region contains seven Ig-like domains capable of binding sialic acid–containing ligands, while the intracellular region includes six tyrosine residues within ITIM and activation-like motifs. These motifs are phosphorylated upon B-cell receptor engagement, regulating both positive and negative B-cell receptor signaling.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested by flow cytometry using normal human peripheral blood cells. Recommended usage: 5 µL (0.02 µg) per test in 100 µL final volume, with cell counts between 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for compatibility.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
• Add 5 µL of CellBlox Plus per sample before combining with cells.
• For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, employing nucleic acid dye structures that enable engineered fluorescence with minimized spillover and spectral spread.

Excitation: 496 nm
Emission: 555 nm
Laser: 488 nm (Blue)

Target Information

CD22 (BL-CAM) is a type I integral membrane glycoprotein (130–140 kDa) expressed on B-lymphocytes. It appears early in B-cell differentiation, similar to CD19, but its membrane expression is limited to mature B cells (CD22⁺) and absent in plasma cells (CD22⁻).
CD22 is strongly expressed in hairy cell leukemia, diffuse large B-cell lymphoma, and nodular lymphocyte-predominant Hodgkin’s lymphoma, but negative in classical Hodgkin’s lymphoma.

It preferentially binds α2,6-linked sialic acid. Ligand-induced tyrosine phosphorylation of CD22 is involved in B-cell receptor signaling regulation, acting both as a positive regulator (via Src family tyrosine kinases) and as an inhibitory receptor (via recruitment of cytoplasmic phosphatases).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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