Thermo Fisher Scientific CD22 Monoclonal Antibody (eBio4KB128 (4KB128)), NovaFluor Blue 555, eBioscience

Applications and Tested Dilution

Flow Cytometry (Flow)

• Tested Dilution: 5 µL (0.2 µg)/test

Product Specifications

Available Formats

• Unconjugated
• APC
• FITC
• NovaFluor Blue 690
• NovaFluor Red 755
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The eBio4KB128 monoclonal antibody recognizes human CD22 (Siglec-2), a member of the siglec subgroup of the Ig superfamily.
CD22 is a type I transmembrane glycoprotein composed of two polypeptide chains (130 and 140 kDa) produced by alternative splicing. It is highly expressed on mature B cells and B cell lymphomas.

The extracellular portion contains seven Ig-like domains capable of binding sialic acid ligands, while the intracellular region includes six tyrosine residues within ITIM and activation-like motifs that regulate B-cell receptor signaling.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells.
Recommended usage: 5 µL (0.02 µg) per test in 100 µL final volume (10⁵–10⁸ cells/test).

Important Notes

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) must be used for optimal staining.
  • Add 5 µL per sample to antibody cocktails before combining with cells.
  • For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures optimized for minimal spillover and spread.

• Excitation: 496 nm
• Emission: 555 nm
• Laser: 488 nm (Blue)

Target Information

CD22 (BL-CAM) is a 130–140 kDa type I membrane glycoprotein expressed in the cytoplasm and membrane of B-lymphocytes.
It appears early in B-cell differentiation and is useful for phenotyping mature leukemias. CD22 is strongly expressed in hairy cell leukemia and positive in diffuse large B-cell lymphoma and nodular lymphocyte predominance Hodgkin’s lymphoma, but negative in classical Hodgkin’s lymphoma.

CD22 preferentially binds α2,6-linked sialic acid, and its phosphorylation regulates B-cell receptor signaling through both positive and inhibitory pathways.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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