Thermo Fisher Scientific CD22 Monoclonal Antibody (eBio4KB128 (4KB128)), NovaFluor Blue 510, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.2 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• FITC
• NovaFluor Blue 690
• NovaFluor Red 755
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The eBio4KB128 monoclonal antibody recognizes human CD22 (Siglec-2), a member of the siglec subgroup of the Ig superfamily. CD22 is a type I transmembrane glycoprotein composed of two polypeptide chains (CD22α and CD22β, 130 and 140 kDa), produced by alternative splicing of the CD22 gene. CD22 is highly expressed on mature B cells and B cell lymphomas.

The extracellular portion of CD22 contains seven Ig-like domains capable of binding ligands with sialic acid moieties on epithelial, endothelial, B, and T cells. The intracellular region contains six tyrosine residues within ITIM and activation-like motifs that regulate B-cell receptor signaling.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested by flow cytometry using normal human peripheral blood cells. Use at 5 µL (0.02 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) must be used for optimal staining.
• Add 5 µL of CellBlox Plus Blocking Buffer per sample before combining with cells.
• For single-color controls: 5 µL per 100 µL of sample (10³–10⁸ cells).

NovaFluor conjugates are based on Phiton™ technology, utilizing nucleic acid dye structures engineered for optimized fluorescence with minimal spillover.

Excitation: 496 nm
Emission: 511 nm
Laser: 488 nm (Blue)

Target Information

CD22 (BL-CAM) is a 130–140 kDa type I membrane glycoprotein expressed on B-lymphocytes. It appears early in B-cell differentiation and is limited to mature B cells (CD22⁺) and absent in plasma cells (CD22⁻). CD22 is strongly expressed in hairy cell leukemia and positive in diffuse large B-cell lymphoma and nodular lymphocyte predominance Hodgkin’s lymphoma, but negative in classical Hodgkin’s lymphoma.

CD22 binds preferentially to α2,6-linked sialic acid. Its phosphorylation upon ligand binding regulates B-cell receptor signaling, acting both as a positive and negative modulator via interactions with Src family kinases and cytoplasmic phosphatases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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