Thermo Fisher Scientific Granulysin Monoclonal Antibody (eBioDH2 (DH2)), PE, eBioscience

Thermo Fisher Scientific Granulysin Monoclonal Antibody (eBioDH2 (DH2)), PE, eBioscience™

Applications

• Intracellular staining
• Flow cytometry
• Immunocytochemistry

Tested Dilution: 5 µL (0.25 µg)/test
Publications: View 5 publications

Product Specifications

Product Specific Information

The eBioDH2 monoclonal antibody reacts with human granulysin, a protein involved in the cytolytic pathway. Granulysin is a member of the saposin-like protein (SAPLIP) family. Two proteins, 9 and 15 kDa, are thought to be post-translationally modified versions of the 15 kDa protein. Granulysin is found in NK and activated T cells, localized to cytolytic granules containing perforin and granzyme. Granulysin and perforin may act synergistically to kill bacteria and induce apoptosis in nucleated cells.

Applications Reported:
This antibody has been reported for use in intracellular staining followed by flow cytometric analysis and immunocytochemistry.

Applications Tested:
Pre-titrated and tested by intracellular staining and flow cytometric analysis of human peripheral blood cells. Recommended usage: 5 µL (0.25 µg) per test in a final volume of 100 µL. Cell number can range from 10⁵ to 10⁸ cells/test.

Excitation: 488–561 nm
Emission: 578 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

GNLY (Granulysin) is a member of the saposin-like protein (SAPLIP) family. It is located in cytotoxic granules of T cells and NK cells, released upon antigen stimulation. GNLY exhibits antimicrobial activity against Mycobacterium tuberculosis and other organisms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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