Thermo Fisher Scientific Podocalyxin Monoclonal Antibody (B34D1.3), PE, eBioscience
인간 Podocalyxin을 인식하는 단클론 항체로, 줄기세포 및 암세포 연구에 적합합니다. PE 형광표지로 유세포분석(Flow Cytometry)에 최적화되어 있으며, 5 µL/test로 사용 가능합니다. 4°C 보관, 동결 금지.
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Applications
- Tested Application: Flow Cytometry (Flow)
- Recommended Dilution: 5 µL (0.5 µg)/test
- Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | B34D1.3 |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation/Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10734225 |
Product Specific Information
The monoclonal antibody B34D1.3 recognizes human podocalyxin, a protein expressed on undifferentiated human embryonic stem cells (ES), embryonic carcinoma cells (EC), and embryonic germ cells (EG). Podocalyxin is a heavily glycosylated cell adhesion protein with homology to CD34 and endoglycan. It was first identified on kidney podocytes and later found to be upregulated in various human cancers such as breast and prostate cancer. The epitope recognized by B34D1.3 is lost upon cell differentiation.
B34D1.3 recognizes the ectodomain of podocalyxin, distinct from the epitopes recognized by Tra-1-60 and Tra-1-81 antibodies. Tra-1-60 reacts with a sialidase-sensitive epitope, while the Tra-1-81 epitope remains undefined.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated and tested on NTERA cell line
- Recommended use: 5 µL (0.5 µg) per test
- A test is defined as the amount of antibody to stain a cell sample in 100 µL final volume
- Cell number range: 10⁵–10⁸ cells/test
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible Lasers: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
This gene encodes a member of the sialomucin protein family, originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane.
Additional biological activities include:
- Binding in a membrane protein complex with Na⁺/H⁺ exchanger regulatory factor to cytoskeletal elements
- Playing a role in hematopoietic cell differentiation
- Expression in vascular endothelium and binding to L-selectin
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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