Thermo Fisher Scientific SSEA3 Monoclonal Antibody (eBioMC-631 (MC-631)), PE, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rat / IgM

Recommended Isotype Control

Rat IgM Isotype Control (eBRM), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioMC-631 (MC-631)

Conjugate

PE PE PE

View additional formats

• Unconjugated
• Alexa Fluor 488
• Biotin
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10854121

Product Specific Information

Description: The eBioMC-631 (MC-631) monoclonal antibody reacts with the carbohydrate moiety galactosylgloboside on Stage-specific embryonic antigen-3 (SSEA-3). Expression is restricted to the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES) and down regulated following differentiation.

Applications Reported: This eBioMC-631 (MC-631) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioMC-631 (MC-631) antibody has been pre-titrated and tested by flow cytometric analysis of the 2102Ep cell line. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

SSEA-3 (stage-specific embryonic antigen 3) is a cell surface antigen, expressed along with SSEA-4, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.