Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), Brilliant Ultra Violet 563, eBioscience

Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), Brilliant Ultra Violet 563, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 615, 661, 737, 805
• Brilliant Violet 421, 605
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue 510, 585
• NovaFluor Yellow 590, 660, 730
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. It exhibits potent immunosuppressive properties and has been proven effective in treating renal, heart, and liver allograft rejection. CD3 is expressed by thymocytes and all mature T cells, playing a key role in TCR complex assembly and signal transduction leading to cellular activation and proliferation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and validated for flow cytometry using normal human peripheral blood cells.
  Recommended use: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Brilliant Ultra Violet™ 563 (BUV563) emits at 564 nm and is optimized for cytometers equipped with a 355 nm ultraviolet laser. Ensure instrument compatibility before use.

Staining Buffer Recommendations

When using multiple polymer dye-conjugated antibodies, employ:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Handling and Fixation

• Light sensitive: protect from photo-induced oxidation.
• Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark.
• Excitation: 350 nm; Emission: 564 nm; Laser: Ultraviolet.

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

The CD3 subunit complex transduces antigen-recognition signals into T cell cytoplasm and regulates TCR surface expression. CD3 subunits (gamma, delta, epsilon, zeta) are part of the immunoglobulin superfamily encoded on chromosome 11. These subunits associate with signal transduction molecules via tyrosine-based motifs. CD3 is expressed on 68–82% of normal peripheral blood lymphocytes and 65–85% of thymocytes, absent on B or NK cells. CD3 gene defects are linked to immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures.

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