Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), Brilliant Ultra Violet 563, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), Brilliant Ultra Violet 563, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506, 570, 615
• FITC
• NovaFluor Blue 585, 610-70S, 660-120S, 760
• NovaFluor Yellow 590, 610, 660
• Pacific Orange
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by thymocytes, mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and plays an essential role in T cell development and function. RM4-5 binding is blocked by GK1.5.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes at ≤0.25 µg/test (100 µL final volume). Adjust cell number empirically (10⁵–10⁸ cells/test). Optimize antibody titration for best results.

Fluorochrome Information:
Brilliant Ultra Violet™ 563 (BUV563) emits at 564 nm and is designed for UV (355 nm) laser-equipped cytometers. Ensure instrument compatibility.

Buffer Recommendations:
Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) when combining multiple polymer dye-conjugated antibodies to minimize non-specific interactions.

Light Sensitivity:
Protect vial and stained samples from light to prevent photo-induced oxidation.

Fixation:
Samples can be fixed in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C (dark). Clone-specific performance should be verified empirically.

Excitation: 350 nm
Emission: 564 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

The CD4 antigen recognizes MHC class II molecules and acts as a co-receptor for HIV. It is primarily expressed on T helper cells and also found on monocytes, macrophages, dendritic cells, and in tissues such as thymus, lymph nodes, tonsils, and spleen. CD4 contributes to T-cell activation via association with Lck kinase and may mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts are known (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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