Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), Brilliant Ultra Violet 563, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 661, 737
• Brilliant Violet 480, 605
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available

Product Specific Information

The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, subsets of mature T cells, and dendritic cells. CD4 binds MHC class II on antigen-presenting cells and plays a critical role in T cell development and function. Binding of GK1.5 is blocked by RM4-5.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes; ≤0.5 µg/test (100 µL final volume)
• Recommended: Titrate antibody for optimal performance

Fluorochrome Information:
Brilliant Ultra Violet™ 563 (BUV563) emits at 564 nm and is intended for use with UV (355 nm) laser-equipped cytometers. Ensure instrument compatibility.

Staining Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to reduce non-specific interactions.

Light Sensitivity:
Protect from light to prevent photo-induced oxidation.

Fixation:
Samples may be stored up to 3 days at 4°C in IC Fixation Buffer (00-8222-49) or 1-Step Fix/Lyse Solution (00-5333-54) with minimal impact on brightness and FRET efficiency.

Excitation/Emission: 350 nm / 564 nm
Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD4 is a co-receptor for MHC class II molecules and plays a key role in T-cell activation. It is primarily expressed on T helper cells and also found on monocytes, macrophages, and dendritic cells. Tissue expression includes thymus, lymph nodes, tonsils, spleen, and specific regions of brain and gut. CD4 associates with Lck tyrosine kinase and may mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts exist (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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