Thermo Fisher Scientific UBA52 Monoclonal Antibody (OTI7G6), TrueMAB
Human UBA52 단백질(aa 77-128)을 항원으로 제작된 Mouse monoclonal antibody (Clone OTI7G6). Western blot에 최적화된 TrueMAB 형식 항체로, PBS buffer에 동결건조되어 제공되며, -20°C에서 보관. 연구용 시약으로 단백질 분해 관련 연구에 적합.
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Applications
- Western Blot (WB): 1:2,000 dilution tested
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI7G6 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 77–128 of human UBA52 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Ubiquitin is a highly conserved protein involved in targeting cellular proteins for degradation via the 26S proteasome.
The UBA52 gene encodes a fusion protein consisting of ubiquitin (N-terminus) and ribosomal protein S27a (C-terminus).
Upon expression, the protein is processed post-translationally to generate free ubiquitin monomer and ribosomal protein S27a.
Ribosomal protein S27a is part of the 40S ribosomal subunit, contains C4-type zinc finger domains, and is localized in the cytoplasm.
Pseudogenes derived from this gene exist in the genome.
Similar fusion proteins include ribosomal protein L40 (with ubiquitin) and ribosomal protein S30 (with ubiquitin-like protein fubi).
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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