Thermo Fisher Scientific UBA52 Monoclonal Antibody (OTI3C1), TrueMAB
Human UBA52 단백질(aa 77-128)에 특이적인 Mouse Monoclonal Antibody. Western blot에 최적화되어 있으며, 1:2,000 희석 비율로 검증됨. Lyophilized 형식으로 제공되며, 재구성 후 약 1 mg/mL 농도. 연구용으로 단백질 분해 조절 연구에 활용 가능.
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Applications
- Western Blot (WB): Tested dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3C1 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 77–128 of human UBA52 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Ubiquitin is a highly conserved protein involved in targeting cellular proteins for degradation by the 26S proteasome.
The UBA52 gene encodes a fusion protein composed of ubiquitin (N-terminus) and ribosomal protein S27a (C-terminus).
Upon expression, the precursor is processed to generate free ubiquitin and ribosomal protein S27a.
Ribosomal protein S27a is part of the 40S ribosomal subunit and contains C4-type zinc finger domains localized in the cytoplasm.
Pseudogenes of this gene exist in the genome.
Similar fusion proteins include ribosomal proteins L40 and S30, which are synthesized with ubiquitin or ubiquitin-like proteins.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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