Thermo Fisher Scientific NR1H3 Monoclonal Antibody (OTI1A5), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

NR1H3 (oxysterols receptor LXR-alpha) is a nuclear receptor with ligand-dependent transcriptional activation activity.
Its interaction with retinoic acid receptor (RXR) converts RXR from a silent DNA-binding partner to an active ligand-binding subunit, mediating retinoid responses via LXRES.
LXRES are DR4-type response elements composed of direct repeats of two similar hexanucleotide half-sites spaced by four nucleotides.
NR1H3 is crucial for cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR, and LRP8.
It also interacts functionally with RORA to regulate genes involved in liver metabolism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.