Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (390), NovaFluor Blue 660-120S, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

• Unconjugated
• APC
• Biotin
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 711
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Red 685 / 710 / 725 / 755
• NovaFluor Yellow 610
• PE
• PE-Cyanine5 / PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645
• Custom conjugation available

Product Specific Information

The 390 monoclonal antibody reacts with mouse CD31 (PECAM-1, gpIIa), a 130–140 kDa surface protein expressed on endothelial cells, leukocytes, and platelets. CD31 mediates homotypic adhesion and is involved in cell-cell and cell-matrix interactions and signal transduction.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported:
Flow cytometry and immunohistochemistry (frozen tissue sections).

Applications Tested:

• Flow cytometry: ≤ 0.6 µg/test (in 100 µL final volume, 10⁵–10⁸ cells/test)
• Immunohistochemistry (frozen tissue): ≤ 20 µg/mL
  Antibody should be titrated for optimal performance.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes such as propidium iodide, 7-AAD, or DAPI.
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for compatibility.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) must be used for optimal staining.
  • Add 5 µL per sample to antibody cocktails before combining with cells.
  • For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates are based on Phiton™ technology, utilizing novel nucleic acid dye structures designed for optimized fluorescence performance with minimal spillover and spread.

Excitation: 509 nm
Emission: 665 nm
Laser: 488 nm (Blue)

Target Information

CD31 (PECAM-1) is an inhibitory coreceptor regulating T and B cell signaling via ITIM motifs. It is expressed throughout the vascular system, mainly at cell junctions, and mediates homophilic binding essential for cell-cell interactions. CD31 plays roles in integrin-mediated adhesion, transendothelial migration, angiogenesis, apoptosis, immune regulation, and thrombosis.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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