Thermo Fisher Scientific IgM Monoclonal Antibody (II/41), NovaFluor Red 710, eBioscience

Thermo Fisher Scientific IgM Monoclonal Antibody (II/41), NovaFluor Red 710, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution

• ≤ 1.0 µg/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant Violet 605
• Brilliant Violet 711
• eFluor 450
• eFluor 570
• eFluor 660
• FITC
• NovaFluor Yellow 610
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 780
• Request custom conjugation

Product Specific Information

The II/41 monoclonal antibody reacts with the mu heavy chain of mouse IgM and does not cross-react with other mouse immunoglobulin classes (IgD, IgG, IgA). IgM is expressed intracellularly in early B lymphopoiesis and on mature B cell surfaces. Fluorochrome-conjugated II/41 can be used for detection of mouse IgM.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse bone marrow cells at ≤1.0 µg/test (in 100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Optimize titration for best performance.

Compatibility Notice:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

CellBlox Plus Blocking Buffer:
This updated NovaFluor conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01). Add 5 µL per sample before combining with cells for optimal staining. For single-color controls, use 5 µL per 100 µL of cell sample.

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures optimized for minimal spillover and spread.

Excitation/Emission:
Excitation: 639 nm
Emission: 710 nm
Laser: 633–640 nm (Red)

Target Information

Mouse IgM is the first antibody produced during an immune response and plays a key role in innate immunity. It consists of five heavy and five light chains, enabling complement activation and antigen agglutination. Widely used in ELISA, Western blotting, and immunohistochemistry for research and diagnostic applications.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

제품 이미지