Thermo Fisher Scientific CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), NovaFluor Red 725, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.8 µg)/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 737
• Brilliant Violet 650
• eFluor 450
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 700
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The eBio124-1D1 monoclonal antibody reacts with human CD7 (gp40, Leu9), a 40 kDa receptor belonging to the immunoglobulin gene superfamily.

• The N-terminal region (aa 1–107) is homologous to Ig kappa light chain, while the C-terminal region is proline-rich.
• CD7 is expressed on most immature and mature T lymphocytes, T cell leukemias, NK cells, and a small subset of normal and malignant B cells.
• Cross-linking of CD7 modulates T cell and NK cell activity, influencing calcium flux, adhesion molecule expression, cytokine secretion, and proliferation.
• CD7 associates with phosphoinositol 3'-kinase and induces D-3 phosphoinositide production and tyrosine phosphorylation.

A clonogenic subpopulation of CD34(+) CD38(-) cord blood cells expressing high CD7 levels has been identified, capable of generating B cells, NK cells, and dendritic cells but not myeloid or erythroid cells. The CD7(+) phenotype distinguishes primitive lymphoid progenitors from pluripotent stem cells.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported

• Suitable for flow cytometric analysis

Applications Tested

• Tested on normal human peripheral blood cells
• Recommended use: ≤5 µL (0.8 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)
• Titrate for optimal performance

Important Notes

• NovaFluor dyes are incompatible with DNA intercalating viability dyes such as propidium iodide, 7-AAD, and DAPI.
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) with NovaFluor conjugates for optimal staining.
  • Add 5 µL per sample, regardless of panel complexity.
  • For single-color controls, use 5 µL per 100 µL of cell sample.

NovaFluor Dye Technology

NovaFluor conjugates utilize Phiton™ technology, employing nucleic acid dye structures designed for engineered fluorescent signatures with minimized spillover and spread.

• Excitation: 636 nm
• Emission: 727 nm
• Laser: 633–640 nm (Red)

Target Information

CD7 (gp40, Leu9) is a 40 kDa immunoglobulin superfamily member expressed on most T lymphocytes, T cell leukemias, NK cells, and subsets of B cells.
It modulates immune cell activation and is a key marker in leukemia diagnostics.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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