Thermo Fisher Scientific CD101 Monoclonal Antibody (Moushi101), NovaFluor Blue 660-120S, eBioscience

Applications

• Flow Cytometry (Flow): Tested at ≤ 0.25 µg/test
  (Recommended to titrate for optimal performance)

Product Specifications

Additional Formats

• Alexa Fluor 700
• APC
• NovaFluor Blue 690
• NovaFluor Yellow 730
• PE
• PE-Cyanine7
• Custom conjugation available

Product Specific Information

The monoclonal antibody Moushi101 recognizes mouse CD101, also known as IGSF2 and V7 (7 IgV domains).
CD101 is a transmembrane glycoprotein with 7 Ig-like domains and a cytoplasmic tail containing several phosphorylation sites.
Cross-linking CD101 can antagonize TCR/CD3-mediated lymphocyte activation.

Expression:

• Monocytes, granulocytes, dendritic cells, Langerhans cells, and activated T cells
• Subset of CD25⁺Foxp3⁺ T regulatory cells with high suppressive activity

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes (≤ 0.25 µg/test, 100 µL final volume)

Note: NovaFluor dyes are not compatible with DNA intercalating viability dyes such as propidium iodide, 7-AAD, and DAPI.
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for compatibility.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
Add 5 µL per sample (regardless of NovaFluor count) before combining with cells.
For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, providing engineered fluorescent signatures optimized for minimal spillover and spread.

• Excitation: 509 nm
• Emission: 665 nm
• Laser: 488 nm (Blue Laser)

Learn more about NovaFluor dyes

Target Information

CD101 (V7 antigen) is a disulfide-linked homodimer belonging to the immunoglobulin superfamily.
It is expressed on monocytes, granulocytes, dendritic cells, and activated T lymphocytes.

• CD101⁺CD28⁺ cells show strong responsiveness to CD28 signaling.
• In combination with anti-CD28 or low anti-CD3, anti-CD101 can enhance proliferation.
• Mouse CD101 is found on a subpopulation of CD4⁺CD25⁺Foxp3⁺ regulatory T cells with high suppressor activity.
• In human PBMCs, ~30% of Foxp3⁺ cells express CD101, though suppressor activity is not fully confirmed.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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