Thermo Fisher Scientific CD41a Monoclonal Antibody (eBioMWReg30 (MWReg30)), NovaFluor Yellow 660, eBioscience

Thermo Fisher Scientific CD41a Monoclonal Antibody (eBioMWReg30 (MWReg30)), NovaFluor Yellow 660

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.6 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 555
• NovaFluor Blue 660-120S
• NovaFluor Yellow 570
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Custom conjugation available

Product Specific Information

The eBioMWReg30 monoclonal antibody reacts with mouse CD41 (fibrinogen receptor, gpIIb, integrin alpha IIb). CD41 is expressed on platelets, megakaryocytes, and hematopoietic progenitors. It associates with CD61 to form a receptor crucial for platelet function, mediating adhesion to fibrinogen, fibronectin, and vitronectin.

Recent studies show that CD150⁺CD48⁻CD41⁻ bone marrow cells efficiently identify hematopoietic stem cells (HSCs).

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse platelets, ≤ 0.125 µg/test (100 µL final volume). Recommended to titrate for optimal performance.

Note:

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining. Add 5 µL per sample (10³–10⁸ cells/test).

NovaFluor conjugates utilize Phiton™ technology, providing engineered fluorescent signatures with reduced spillover.
Excitation: 552 nm; Emission: 663 nm; Laser: 561 nm (Yellow).

Strain-specific note: Non-specific staining of B cells observed in SJL mice; not in BALB/c or C57BL/6.

Target Information

CD41 (platelet glycoprotein IIb, ITGA2B) forms a receptor with CD61, mediating platelet aggregation and adhesion to von Willebrand factor, fibrinogen, fibronectin, and vitronectin. Expressed on megakaryocyte-platelet lineage and early hematopoietic cells. Associated disorders include Glansmann Thrombasthenia and Platelet type-16 bleeding disorder.

For Research Use Only. Not for use in diagnostic procedures.

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