Thermo Fisher Scientific M-CSF Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific M-CSF Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

• Western Blot (WB): 0.1–0.2 µg/mL
• ELISA: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human M-CSF. Anti-Human M-CSF-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hM-CSF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human M-CSF (500-P44) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hM-CSF.

Western Blot:
To detect hM-CSF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
Used with compatible secondary reagents, the detection limit for Recombinant hM-CSF is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P44BT-1MG will be provided as 2 × 500 µg.

Target Information

M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells.
It induces VEGF secretion by macrophages, mediating mobilization of endothelial progenitor cells and neovascularization.
M-CSF exists as several bioactive isoforms differing in potency and stability, synthesized as a membrane-spanning protein that can be cleaved and modified in the secretory vesicle.
It forms a disulfide-bonded homodimer processed into glycoprotein or proteoglycan isoforms.
Binding to its receptor c-Fms (CSF-1R or CD115) induces receptor dimerization, internalization, and degradation.
Functionally, M-CSF stimulates differentiation of hematopoietic stem cells to monocyte-macrophage populations.
Human M-CSF shows activity on mouse cells, while mouse CSF shows no activity on human cells.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

제품 이미지

(이미지 파일: 500-P44BT-25UG_M-CSF_P09603-1_Rabbit.svg, 500-P44BT-25UG_M-CSF_P09603-1_Rabbit_PDP.jpeg)