Thermo Fisher Scientific G-CSF Polyclonal Antibody, PeproTech

Thermo Fisher Scientific G-CSF Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human G-CSF. Anti-Human G-CSF-specific antibody was purified by affinity chromatography employing an immobilized Human G-CSF matrix.

Sandwich ELISA:
To detect Human G-CSF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human G-CSF (500-P43Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human G-CSF.

Western Blot:
To detect hG-CSF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant hG-CSF is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Target Information

G-CSF (Granulocyte Colony Stimulating Factor) is a naturally occurring cytokine that stimulates the production and antibacterial function of neutrophils and monocytes.
Human G-CSF is an 18.8 kDa protein containing 175 amino acid residues, and a soluble isoform of the G-CSF receptor has been described.

The pleiotropic cytokine is produced by activated monocytes, macrophages, endothelial cells, fibroblasts, astrocytes, osteoblasts, and bone marrow cells. G-CSF has specific effects on the proliferation, differentiation, and activation of hematopoietic cells. It is also expressed by various transformed cells such as carcinoma and myeloblastic leukemia cells.

G-CSF is encoded by two distinct DNA sequences, resulting in a full-size, high-activity and a shorter, low-activity isoform. It is highly conserved among species and exerts its biological functions through interaction with its receptor on hematopoietic progenitors, neutrophilic granulocytes, and certain carcinoma cell lines.

Clinical use of G-CSF includes treatment of neonatal infections, acute myocardial infarction, granulocyte transfusion in neutropenia, severe infections, sepsis, chronic autoimmune neutropenia, acute myeloid leukemias, Sweet’s syndrome, and AIDS.
G-CSF also serves as a marker protein for various carcinomas and is associated with Kostmann Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.