Thermo Fisher Scientific EGF Polyclonal Antibody, PeproTech
Rabbit polyclonal antibody against Human EGF, validated for WB and ELISA. Affinity purified, lyophilized form, stored at -20°C. Detects recombinant EGF with high sensitivity; ideal for research on EGFR signaling pathways.
- 카탈로그번호
- 500-P45-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific EGF Polyclonal Antibody, PeproTech
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA (ELISA) | 0.5–2.0 µg/mL | - |
| In vitro Assay (IV) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human EGF |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930551 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human EGF. Anti-Human EGF-specific antibody was purified by affinity chromatography employing an immobilized Human EGF matrix.
Sandwich ELISA:
To detect Human EGF by sandwich ELISA (100 µL/well antibody solution), use 0.5–2.0 µg/mL of this antibody. When used with PeproTech Biotinylated Anti-Human EGF (500-P45Bt) as a detection antibody, it can detect at least 0.2–0.4 ng/well of Recombinant Human EGF.
Western Blot:
For detection of Human EGF by Western Blot, use 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human EGF is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
EGF (Epidermal Growth Factor):
EGF binds to the EGF receptor (EGFR), a 170 kDa protein kinase, initiating multiple cellular pathways in response to environmental stimuli. Upon binding, EGFR forms homo- or heterodimers with other family members, triggering distinct signaling cascades via SH2-containing effector proteins. EGF is widely expressed in kidney, cerebrum, prostate, and salivary glands, acting as a potent mitogenic factor. It activates major pathways including RAS-RAF-MEK-ERK, PI3K-AKT, PLCγ-PKC, and STAT. Dysregulation of EGF signaling is associated with tumor progression and certain genetic disorders such as hypomagnesemia type 4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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