Thermo Fisher Scientific M-CSF Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL
• Publications: 1 publication available

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human M-CSF. Anti-Human M-CSF-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hM-CSF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human M-CSF (500-P44) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hM-CSF.

Western Blot:
To detect hM-CSF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hM-CSF is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Packaging:
500-P44BT-1MG will be provided as 2 × 500 µg.

Target Information

M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. It induces VEGF secretion by macrophages, mediating mobilization of endothelial progenitor cells and neovascularization.
M-CSF exists as several bioactive isoforms differing in potency and stability. The full-length protein is a membrane-spanning form that can be expressed on the cell surface or cleaved and modified in the secretory vesicle.
It is a disulfide-bonded homodimer processed into either a glycoprotein or a proteoglycan modified by chondroitin sulfate addition. Binding of M-CSF to its receptor c-Fms (CSF-1R/CD115) induces receptor dimerization, internalization, and degradation. Functionally, M-CSF stimulates differentiation of hematopoietic stem cells into monocyte-macrophage populations.
Human M-CSF shows activity on mouse cells, but mouse CSF shows no activity on human cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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