Thermo Fisher Scientific G-CSF Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human G-CSF. Anti-Human G-CSF-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hG-CSF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human G-CSF (500-P43) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hG-CSF.

Western Blot:
To detect hG-CSF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hG-CSF is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package Information:
500-P43BT-1MG will be provided as 2 × 500 µg.

Target Information

G-CSF (Granulocyte Colony Stimulating Factor)
G-CSF is a naturally occurring cytokine that stimulates the production and antibacterial function of neutrophils and monocytes. Human G-CSF is an 18.8 kDa protein containing 175 amino acid residues, and a soluble isoform of the G-CSF receptor has been described. This cytokine affects the proliferation, differentiation, and activation of hematopoietic cells and is produced by monocytes, macrophages, endothelial cells, fibroblasts, astrocytes, osteoblasts, and bone marrow cells.

G-CSF is expressed by various transformed cells such as carcinoma and myeloblastic leukemia cells. It is encoded by two distinct DNA sequences, resulting in a full-size, high-activity and a shorter, low-activity isoform. G-CSF functions through interaction with its receptor on hematopoietic progenitors, neutrophilic granulocytes, and certain carcinoma cell lines.

Clinically, G-CSF is used in treatments for neonatal infections, acute myocardial infarction, neutropenia, severe infections, sepsis, chronic autoimmune neutropenia, acute myeloid leukemia, Sweet’s syndrome, and AIDS. Dysfunction of G-CSF is linked to conditions such as Kostmann Syndrome and certain carcinomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.