Thermo Fisher Scientific PCNA Monoclonal Antibody (OTI5F9), TrueMAB

Applications

• Western Blot (WB)
  Tested Dilution: 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and participates in DNA excision and mismatch repair pathways. It interacts with CDK inhibitor p21, endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT).
PCNA serves as a marker for proliferating cells and tumor tissue prognosis, being expressed in early G1 and S phases. Located in the nucleus, PCNA acts as a cofactor for DNA polymerase delta, forming a homotrimer to enhance leading strand synthesis.
Upon DNA damage, PCNA undergoes ubiquitination and functions in the RAD6-dependent repair pathway. Two transcript variants encoding the same protein exist, and pseudogenes are present on chromosome 4 and X chromosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.