Thermo Fisher Scientific PCNA Monoclonal Antibody (OTI4G3), TrueMAB

Applications

• Western Blot (WB): 1:2,000
• Immunohistochemistry (Paraffin) (IHC (P)): 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

PCNA (Proliferating Cell Nuclear Antigen) is essential for DNA replication and repair.
It interacts with CDK inhibitor p21, endonucleases Fen1 and XPG, and DNA methyltransferase MCMT.
PCNA serves as a marker for proliferating cells and tumor prognosis, being expressed during early G1 and S phases.
Located in the nucleus, PCNA forms a homotrimer that enhances leading strand synthesis during DNA replication.
Upon DNA damage, PCNA is ubiquitinated and participates in RAD6-dependent repair pathways.
Two transcript variants encoding the same protein are known, and pseudogenes are present on chromosomes 4 and X.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지