Thermo Fisher Scientific PCNA Monoclonal Antibody (OTI4B2), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways.
It interacts with CDK inhibitor p21, endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT).
PCNA serves as a marker for proliferative cells and tumor tissue prognosis.
It is localized in the nucleus, acts as a cofactor of DNA polymerase delta, and functions as a homotrimer to enhance leading strand synthesis during DNA replication.
Upon DNA damage, PCNA is ubiquitinated and participates in the RAD6-dependent DNA repair pathway.
Two transcript variants encoding the same protein have been identified, and pseudogenes are found on chromosome 4 and the X chromosome.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.