Thermo Fisher Scientific PCNA Monoclonal Antibody (OTI2G7), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

PCNA (proliferating cell nuclear antigen) is essential for DNA replication and involved in DNA excision and mismatch repair pathways. It binds to CDK inhibitor p21, structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT).
PCNA serves as a marker for proliferating cells and can indicate tumor proliferation status. It is localized in the nucleus, acts as a cofactor of DNA polymerase delta, and functions as a homotrimer to enhance leading strand synthesis during replication.
Upon DNA damage, PCNA undergoes ubiquitination and participates in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been identified, and pseudogenes exist on chromosome 4 and the X chromosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.