Thermo Fisher Scientific HLA-E Monoclonal Antibody (3D12HLA-E), eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugated Formats

• Alexa Fluor 488: 53-9953-42
• APC: 17-9953-42
• Functional Grade: 16-9953-82
• PE: 12-9953-42
• Custom conjugation available upon request

Product Specific Information

Description:
The monoclonal antibody 3D12 (HLA-E) recognizes human HLA-E, a member of the Human Leukocyte Antigen family. HLA-E is an MHC Class IB molecule involved in NK cell recognition. It binds a restricted set of peptides derived from other Class I leader sequences, forming a complex with β2-microglobulin (B2M) that interacts with NKG2A on NK cells, protecting HLA-G-expressing cells from NK cell-mediated killing. HLA-E expression is ubiquitous.

Applications Tested:
This antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. Recommended usage is ≤1 µg per test (defined as the amount required to stain cells in 100 µL final volume). Optimal titration is advised for specific assays.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

HLA-E belongs to the HLA class I heavy chain paralogues. It forms a heterodimer with β2-microglobulin, with the heavy chain anchored in the membrane. HLA-E binds peptides derived from other class I molecule leader sequences.
The heavy chain (~45 kDa) gene contains 8 exons encoding distinct structural domains:

• Exon 1: Leader peptide
• Exons 2–3: α1 and α2 domains (peptide binding)
• Exon 4: α3 domain
• Exon 5: Transmembrane region
• Exons 6–7: Cytoplasmic tail

HLA and MHC antibodies are essential tools in Immunopeptidomics, aiding in the identification and characterization of neoantigens via HPLC and tandem mass spectrometry.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.