Thermo Fisher Scientific CD278 (ICOS) Monoclonal Antibody (C398.4A), eBioscience
CD278 (ICOS) 단일클론 항체(C398.4A)는 활성화된 T세포의 공동자극 분자인 ICOS를 인식하며, Flow Cytometry, IHC, IP, Functional Assay 등에 사용됩니다. 0.5 mg/mL 농도의 액상 형태로 제공되며, PBS(pH 7.2) 버퍼와 0.09% sodium azide를 포함합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | Assay-Dependent | - |
| Flow Cytometry (Flow) | 0.25 µg/test | 18 publications |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Functional Assay | Assay-Dependent | 2 publications |
| Blocking Assay | - | 1 publication |
| Mass Spectrometry (MS) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rhesus monkey, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | C398.4A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468637 |
Additional Available Formats
- APC (17-9949-82)
- APC-eFluor 780 (47-9949-82)
- Biotin (13-9949-82)
- FITC (11-9949-82)
- Functional Grade (16-9949-82)
- PE (12-9949-81)
- PE-Cyanine5 (15-9949-82)
- PE-Cyanine7 (25-9949-82)
- Super Bright 436 (62-9949-82)
- Request custom conjugation (custom form link)
Product Specific Information
The C398.4A monoclonal antibody reacts with ICOS (Inducible Costimulatory molecule), also known as H4/CRP-1/AILIM, and cross-reacts with mouse and rat ICOS.
ICOS is a T cell-specific activation molecule and a member of the CD28/CTLA-4 family. It is a 47–57 kDa dimer expressed on activated T cells and plays a key role in T cell activation, proliferation, and humoral immune response.
The C398.4A antibody strongly stimulates proliferation of T cell clones and IL-10 production from pre-activated cells. The epitope recognized by C398.4A differs from that bound by ICOSL, meaning it does not block ICOSL binding.
Applications Reported: Flow cytometry, immunoprecipitation, immunohistochemistry, and in vitro functional assays.
Note: Not suitable for Western blot (WB). For functional assays, use Functional Grade purified C398.4A (Product #16-9949).
Applications Tested: Tested by flow cytometric analysis of Con A-stimulated mouse splenocytes at ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Titrate for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
ICOS (CD278) encodes a protein of the CD28/CTLA-4 receptor family, functioning as a homodimer involved in cell signaling, immune responses, and regulation of cell proliferation.
It has been associated with immunodeficiency disorders such as Common Variable Immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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