Thermo Fisher Scientific HLA-G Monoclonal Antibody (87G), eBioscience
인간 HLA-G 단백질을 인식하는 87G 클론 단일클론 항체로, 비고전적 MHC 타입 관련 면역억제 연구에 적합. Flow Cytometry 및 IHC(Frozen) 응용 가능. 고순도(>90%)로 제조되며, 다양한 형광 표지 형식 제공. 연구용으로만 사용.
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Thermo Fisher Scientific HLA-G Monoclonal Antibody (87G), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | – | View 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | – |
| Flow Cytometry (Flow) | 0.5 µg/test | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 87G |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865531 |
Additional Formats Available:
Product Specific Information
The monoclonal antibody 87G recognizes human HLA-G, a nonclassical MHC type involved in inhibiting immune responses.
HLA-G has seven isoforms, and antibody 87G recognizes both HLA-G1 and soluble HLA-G5.
Expression of HLA-G is found primarily in fetal trophoblast cells, protecting the fetus from maternal immune attack.
Abundant HLA-G expression is also identified in certain tumors such as melanoma, breast carcinoma, renal carcinoma, and hematologic malignancies (CLL, AML, B-CLL).
Additional expression is seen in pancreatic islets, erythroid and endothelial progenitors, and adult thymic medulla.
HLA-G⁺ CD4 or CD8 cells in normal peripheral blood act as regulatory cells with a unique cytokine profile.
Receptors for HLA-G include CD85j/ILT2, CD85d/ILT4, and CD158.
Recent studies show a role for HLA-G in transplant tolerance and maintenance.
Applications Reported:
- Flow cytometric analysis
- Immunohistology staining of frozen tissue sections
Applications Tested:
- Tested by flow cytometric analysis of normal human peripheral blood
- Effective at ≤ 0.5 µg per test (100 µL final volume)
- Recommended to titrate for optimal assay performance
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
HLA-G belongs to the HLA class I heavy chain paralogues.
It forms a heterodimer of a heavy chain and beta-2 microglobulin.
The heavy chain (~45 kDa) gene contains 8 exons:
- Exon 1: Leader peptide
- Exons 2–3: α1 and α2 domains (peptide binding)
- Exon 4: α3 domain
- Exon 5: Transmembrane region
- Exon 6: Cytoplasmic tail
HLA-G is expressed on fetal-derived placental cells and plays a key role in immune tolerance.
HLA/MHC antibodies are crucial for Immunopeptidomics, enabling identification and characterization of neoantigens via LC-MS/MS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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