Thermo Fisher Scientific Blimp-1 Monoclonal Antibody (6D3), eBioscience

Thermo Fisher Scientific Blimp-1 Monoclonal Antibody (6D3), eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

This 6D3 monoclonal antibody reacts with B lymphocyte-induced maturation protein-1 (Blimp-1), a transcriptional repressor expressed in B and T cells.
Blimp-1 (~90 kDa) contains five Kruppel-like zinc finger domains for DNA binding.
In B cells, it promotes terminal differentiation into antibody-secreting plasma cells during immune responses.
It is also involved in germinal center formation and certain diffuse large B cell lymphomas.
Expressed in CD4⁺, CD8⁺ effector/memory T cells, and FoxP3⁺ regulatory T cells, Blimp-1 contributes to T cell homeostasis, IL-2 suppression, and Th1 differentiation regulation.
Additionally, Blimp-1 is critical for effector and effector memory CD8⁺ T cell differentiation during viral infection.

Applications Reported: Immunoblotting (WB) and immunohistology staining of paraffin-embedded tissue sections.
Applications Tested:

• Western blot of reduced A20 cell lysates (1–10 µg/mL).
• Immunohistochemistry of formalin-fixed paraffin-embedded human tonsil tissue (10 µg/mL, antigen retrieval with 10 mM citrate buffer, pH 6.0).
  Careful titration is recommended for optimal assay performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its C-terminus.
It acts as a transcriptional repressor essential for terminal differentiation of B lymphocytes and monocyte/macrophages.
Blimp-1 expression is also observed in epithelial cells, retinal neurons, and other differentiated lineages.
It participates in repression of IFN-beta and c-myc gene expression.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.