Thermo Fisher Scientific Blimp-1 Monoclonal Antibody (6D3), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

1-10 µg/mL

View 8 publications 8 publications

Immunohistochemistry (IHC)

-

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 4 publications 4 publications

Flow Cytometry (Flow)

(0.5 µg)/test)

View 1 publication 1 publication

ChIP assay (ChIP)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

6D3

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1907438

Product Specific Information

Description: This 6D3 monoclonal antibody reacts with B lymphocyte-induced maturation protein-1 (Blimp-1), a transcriptional repressor expressed in B and T cells. This approximately 90-kDa protein contains five consecutive Kruppel-like zinc finger domains by which it binds DNA. In B cells, Blimp-1 promotes terminal differentiation into antibody-secreting plasma cells during a primary immune response. In addition, Blimp-1 has been implicated in germinal center formation in response to T-dependent and T-independent antigens, as well as in some diffuse large B cell lymphomas. Expressed in CD4+ and CD8+ effector/memory T cells and FoxP3+ regulatory T cells, reports have suggested possible roles for Blimp-1 in T cell homeostasis and suppression of IL-2 production. Moreover, by suppressing Th1 differentiation, Blimp-1 plays a role in T helper cell differentiation. Finally, Blimp-1 has been demonstrated to play a significant role in the terminal differentiation of effector and effector memory CD8+ T cells during viral infection.

Applications Reported: This 6D3 antibody has been reported for use in immunoblotting (WB) and immunohistology staining of paraffin-embedded tissue sections.

Applications Tested: This 6D3 antibody has been tested by western blot of reduced A20 cell lysates. For western blottiing, this antibody can be used at 1-10 µg/mL. Moreover, this antibody was tested by immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue. Antigen retrieval was performed using 10mM citrate buffer at pH 6.0. For immunohistochemistry, this antibody can be used at 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.